cDNA Cloning and Sequence Analysis of Rice Sbe1 and Sbe3 Genes

作     者：CHENXiu-hua LIUQiao-quan WuHsin-kan WANGZong-yang GuMing-hong CHEN Xiu-hua, LIU Qiao-quan, Wu Hsin-kan, WANG Zong-yang, Gu Ming-hong (Agricultural College, Yangzhou University, Yangzhou 225009, China;College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China;Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China)

作者机构：AgriculturalCollegeYangzhouUniversityYangzhou225009China ShanghaiInstituteofPlantPhysiologyandEcologyChineseAcademyofSciencesShanghai200032China 

出 版 物：《Rice science》 (水稻科学（英文版）)

年 卷 期：2004年第11卷第3期

页      面：81-85页

学科分类：09[农学] 0901[农学-作物学] 

基　　金：国家高技术研究发展计划(863计划)(2001AA212101) High Technique Research Project of Jiangsu Province(BG2001302) 江苏省自然科学基金(DK2001212) 

主　　题：rice starch-branching enzyme genes cDNA sequence gene clone 

摘      要：Two starch-branching enzyme (SBE) in rice, is known to be a key enzyme in amylopectin biosynthesis. The cDNA of two SBE(starch-branching enzyme) genes Sbe1 and Sbc3 encoding SBE Ⅰ and SBE Ⅲ (two major isoforms in rice) were cloned by an improved RT-PCR technique, from a template cDNA library derived from the total mRNAs extracted from the immature seeds of a japonica rice Wuyunjing 7. DNA sequence analysis showed that the size of the cloned Sbe1 and Sbe3 cDNAs were 2490 and 2481 bp long, respectively, including their entire coding sequences. Comparison analysis indicated that the nucleotide sequence of Sbe3 was the same as that of sbc3 (Genbank Accession No. D16201) as reported previously. There were only four base-pairs difference, which resulted in changes of two deduced amino acids between the cloned Sbel cDNA and the reported sbe1 (Genbank Accession No. D11082). The cloned Sbe1 and Sbe3 cDNAs make it possible to improve rice starch quality through genetic engineering