Phytosphinganine Affects Plasmodesmata Permeability via Facilitating PDLP5-Stimulated Callose Accumulation in Arabidopsis
Phytosphinganine 经由在 Arabidopsis 便于刺激 PDLP5 的 Callose 累积影响 Plasmodesmata 渗透作者机构：State Key Laboratory of Protein and Plant Gene ResearchCollege of-Life SciencesPeking UniversityRoom 228Jinguang BuildingNo5 in Yi-He Yuan RoadBeijing 100871People's Republic of China State Key Laboratory of Plant GenomicsInstitute of MicrobiologyChinese Academy of SciencesBeijing 100101 People's Republic of China Institute for Advanced StudiesWuhan UniversityWuhan 430072People's Republic of China
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2020年第13卷第1期
页 面：128-143页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：This research was supported by grants from the National Science and Technology Major Project(2016ZX08010-001) the National Natural Science Foundation of China(31570283)
主 题：plasmodesmata phytosphinganine sphingolipid binding motif PDLP5
摘 要：Plant plasmodesmata (PDs) are specialized channels that enable communication between neighboring cells. The intercellular permeability of PDs, which affects plant development, defense, and responses to stimuli, must be tightly regulated. However, the lipid compositions of PD membrane and their impact on PD permeability remain elusive. Here, we report that the Arabidopsis sld1 sld2 double mutant, lacking sphingolipid long-chain base 8 desaturases 1 and 2, displayed decreased PD permeability due to a significant increase in callose accumulation. PD-located protein 5 (PDLP5) was significantly enriched in the leaf epidermal cells of sld1 sld2 and showed specific binding affinity to phytosphinganine (t18:0), suggesting that the enrichment of t18:0-based sphingolipids in sld1 sld2 PDs might facilitate the recruitment of PDLP5 proteins to PDs. The sld1 sld2 double mutant seedlings showed enhanced resistance to the fungal-wilt pathogen Verticillium dahlia and the bacterium Pseudomonas syringae pv. tomato DC3000, which could be fully rescued in sld1 sld2 pdlp5 triple mutant . Taken together, these results indicate that phytosphinganine might regulate PD functions and cell-to-cell communication by modifying the level of PDLP5 in PD membranes.
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