Influence of Toxoplasma gondii on in vitro proliferation and apoptosis of hepatoma carcinoma H7402 cell

作     者：Gang Wang Ming Gao 

作者机构：Department of EmergencyPeople's Hospital of Zhengzhou Department of Oncologythe First Affiliated Hospital of Zhengzhou University 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2016年第9卷第1期

页      面：60-63页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 100214[医学-肿瘤学] 10[医学] 

主　　题：Toxoplasma gondii Hepatoma carcinoma H7402 cell line Cell proliferation 

摘      要：Objective:To discuss the influence of tachyzoite of Toxoplasma gondii（***） RH strain on proliferation and apoptosis of hepatoma carcinoma（HCC） H7402 ***:The HCC H7402 cell in logarithmic phase and tachyzoite of *** RH strain in different concentrations（1×107/mL,2×107/mL.4×107/mL,8×107mL and 16×107/mL） were ***-8was utilized to determine the inhibition rate of *** tachyzoite on H7402 cell *** cytometry was used to detect the change of cell ***-PCR method was used to detect the expression of cyclinB1 and cdc2-two genes related to cell *** blot method was used to detect the expression of apoptosis-related proteins Caspase-3 and ***:The tachyzoite of *** RH strain can inhibit the proliferation of HCC H7402 *** inhibition rate of tumor cell growth increased with the increase of concentration of *** *** the increase of concentration of *** tachyzoite,the proportion of G0/G1 phase of H7402 cell increased,the proportion of S phase decreased,and PI value decreased *** expression of cyclinB1 and cdc2 genes decreased with the increase of the concentration of *** *** the increase of the concentration of tachyzoite of *** RH strain,the expression quantity of Caspase-3 in H7402 cell increased,but the expression quantity of Bcl-2protein ***:*** can inhibit the in vitro proliferation of HCC H7402 cell,and induce its *** effect shows a trend of concentration-dependent ***,it is related to the down-regulation of cyclinB1 and cdc2（cell cycle-related genes）,the increase of apoptosis-related protein *** the decreasc of Bcl-2 expression.