Protective Effect of Interleukin-1β on Motor Neurons after Sciatic Nerve Injury in Rats

作     者：翁雨雄 巴拉特 洪光祥 王发斌 陈振斌 黄启顺 

作者机构：Department of Hand Surgery 

出 版 物：《Journal of Huazhong University of Science and Technology(Medical Sciences)》 (华中科技大学学报（医学英德文版）)

年 卷 期：2004年第24卷第1期

页      面：71-74页

核心收录：

学科分类：1002[医学-临床医学] 10[医学] 

主　　题：protective effect interleukin 1β motor neurons nerve injury 

摘      要：Protective effect of interleukin 1β (IL 1β) on motor neurons was studied after peripheral nerve injury. Twenty Wistar rats were divided into 2 groups randomly. The right sciatic nerve of each rat was resected. After silicon tubulization of sciatic nerve in rat, 15 μl 1 ng/ml IL 1β and PBS solution were injected into the silicon capsule respectively. Enzyme histochemistry was performed to show acetyle cholesterase (AchE) and nitric oxide staining (NOS) activity of spinal α motor neurons in spinal segments 2 weeks later. Neurons were counted and the diameter and cross sectional (c/s) area of neurons were analyzed by using computer image analysis system. The results showed that as compared with the normal side, both enzyme activities significantly changed in motor neurons in PBS group. The diameter and c/s area of both neurons changed significantly too ( P 0 01). These results suggest that exogenous IL 1β protects α motor neurons from degeneration and necrosis after peripheral nerve injury.