The polysaccharide isolated from Pleurotus nebrodensis(PN-S) shows immune-stimulating activity in RAW264.7 macrophages

作     者：CUI Hai-Yan WANG Chang-Lu WANG Yu-Rong LI Zhen-Jing ZHANG Ya-Nan 

作者机构：Key Laboratory of Food Nutrition and SafetyMinistry of EducationSchool of Food Engineering and BiotechnologyTianjin University of Science and Technology 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2015年第13卷第5期

页      面：355-360页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 0703[理学-化学] 10[医学] 100602[医学-中西医结合临床] 

基　　金：supported by the National Natural Science Foundation of China(Nos.31101357 and 31271915) 

主　　题：Pleurotus nebrodensis Polysaccharide Immunization Cytokines 

摘      要：A novel Pleurotus nebrodensis polysaccharide(PN-S) was purified andcharacterized,and its immune-stimulating activity was evaluated in RAW264.7 macrophages.PN-S induced the proliferation of RAW264.7 cells in a dose-dependent manner,as determined by the MTT assay.After exposure to PN-S,the phagocytosis of the macrophages was significantly improved,with remarkable changes in morphology being observed.Flow cytometric analysis demonstrated that PN-S promoted RAW264.7 cells to progress through S and G2/M phases.PN-S treatment enhanced the productions of interleukin-6(IL-6),nitric oxide(NO),interferon gamma(INF-γ),and tumor necrosis factor-α(TNF-α) in the macrophages,with up-regulation of m RNA expressions of interleukin-6(IL-6),inducible nitric oxide synthase(i NOS),interferon gamma(INF-γ) and tumor necrosis factor-α(TNF-α) being observed in a dose-dependent manner,as measured by q RT-PCR.In conclusion,these results suggest that the purified PN-S can improve immunity by activating macrophages.