Comparative identification of Ca～(2+) channel expression in INS-1 and rat pancreatic β cells

作     者：Fei Li Zong-Ming Zhang 

作者机构：Department of General Surgery Digestive Medical Center The First Affiliated Hospital Medical School Tsinghua University Beijing 100016 China 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2009年第15卷第24期

页      面：3046-3050页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 071002[理学-动物学] 

基　　金：Supported by The Tsinghua-Yue-Yuan Medical Science Fund No20240000568 

主　　题：L-type calcium channels Expression profile Insulin-secreting cells Rats pancreatic β cell Reverse transcription-polymerase chain reaction 

摘      要：AIM： To identify and compare the profile of Ca^2＋ channel subunit expression in INS-1 and rat pancreatic β cells. METHODS： The rat insulin-secreting INS-1 cell line was cultured in RPMI-1640 with Wistar rats employed as islet donors. Ca^2＋ channel subunit expression in INS-1 and isolated rat β cells were examined by reverse transcription polymerase chain reaction （RT-PCR）. Absolute real-time quantitative PCR was performed in a Bio-Rad iQ5 Gradient Real Time PCR system and the data analyzed using an iQ5 system to identify the expression level of the Ca^2＋ channel subunits. RESULTS： In INS-1 cells, the L-type Ca^2＋ channel 1C subunit had the highest expression level and the TPRM2 subunit had the second highest expression. In rat β cells, the TPRC4β subunit expression was dominant and the expression of the L-type lC subunit exceeded the 1D subunit expression about two-fold. This result agreed with other studies, confirming the important role of the L-type lC subunit in insulinsecreting cells, and suggested that non-voltage-operated Ca^2＋ channels may have an important role in biphasic insulin secretion. CONCLUSION： Twelve major Ca^2＋ channel subunit types were identified in INS-1 and rat β cells and significant differences were observed in the expression of certain subunits between these cells.