Gene silencing of MAT1 mediated by siRNA inhibits cell proliferation and invasion in pancreatic cancer in vitro

作     者：Shineng Zhang Jianping Liu Fengqin Xu Yiwen Wanga Wa Zhong Shizhen Yuan 

作者机构：Department of Gastroenterology The Second Affiliated Hospital Sun Yat-sen University Guangzhou 510120 China Department of Nosocomiology The Second Affiliated Hospital Sun Yat-sen University Guangzhou 510120 China Department of Pharmacy The Second Affiliated Hospital Sun Yat-sen University Guangzhou 510120 China 

出 版 物：《The Chinese-German Journal of Clinical Oncology》 (中德临床肿瘤学杂志（英文版）)

年 卷 期：2008年第7卷第5期

页      面：280-282页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：a grant from the Guangdong Science and Technology Commission(No.04009381) 

主　　题：RNA interference cell proliferation invasion pancreatic cancer 

摘      要：Objective： To observe the feasibility of gene silencing of MAT1 gene by small interference RNA in human pan- creatic cancer cells. Methods： BxPC-3 cells were transfected using chemically synthesized double stranded RNA formulated with liposome. Gene expression of MAT1 was assayed by RT-PCR and Western blot respectively. The cell proliferation as- say was carried out by counting alive cells after Trypan blue exclusion. The cells invasion ability was determined by Boyden chamber model. Results： The MAT1 mRNA and protein expression of BxPC-3 cells were significantly down-regulated by small interference RNA compared with the control groups. The expression of MAT1 mRNA was reduced by 55.2% and 64.3% in 24 h and 48 h respectively （P 〈 0.01）. The cell proliferation and invasion ability of BxPC-3 cell were significantly inhibited （P 〈 0.01）. Conclusion： The results suggest that gene silencing of MAT1 by siRNA can inhibit the cell proliferation and invasion of BxPC-3 cells, which may be a target in the gene therapy of human pancreatic cancer.