Determination of ribavirin by flow injection chemiluminescence

作     者：Juqing Jin Yuhai Tang Langchong He Hong Yao Yuanyuan Sun 

作者机构：School of Science . Xi＇an Jiaotong University Xi＇an 710061 China School of Medicine Xi＇an Jiaotong University Xi＇an 710061 China Pharmacy Faculty of Fujian Medical University Fuzhou 350004 China 

出 版 物：《Journal of Nanjing Medical University》 (南京医科大学学报（英文版）)

年 卷 期：2006年第20卷第5期

页      面：266-270页

学科分类：1007[医学-药学(可授医学、理学学位)] 10[医学] 

主　　题：flow injection analysis chemiluminescence ribavirin 

摘      要：Objective： To establish a rapid and precise continuous flow-injection chemiluminescence （CL） method to determine ribavirin. Methods： The ribavirin could restrain strongly CL reaction of luminol in sodium hydroxide （NaOH） solution with potassium chlorate （KClO3）. The different experimental parameters affecting the CL intensity were studied carefully. Results： Under optimum conditions （The concentrations of luminol, KClO3, and NaOH were at 0.1 mmol/L, 0.5μmol/L, and 0.2 mol/L, respectively）, the linear range of the working curves was 0.01-7.00μg/ml with a detection limit of 0.004μg/ml. Conclusion： The method is simple, rapid and sensitive, and successfully applied to the determination of ribavirin in pharmaceutical preparations and biological fluids.