Studies on apoptosis of spermatogenic cells in normal fertile men treated with supraphysiological doses of testosterone undecanoate

作     者：Yi-FengGE Yu-FengHUANG Gui-YuaaZHANG Xinghaiwang jianpingxu 

作者机构：Laboratoryofreproductionandgeneticsgeneralhospitalofnanjingcommandplananjing210002china Nationalresearchinstitudeforfamilyplanningbeijing100018china Jiangsufamtityplanrringresearchinstinutenanjing210029china 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：1999年第1卷第3期

页      面：155-158页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071008[理学-发育生物学] 

主　　题：anti-spermatogenic effect testosterone undecanoate apoptosis germ cells semen 

摘      要：Aim: To study the anti-spermatogenic mechanism of supra-physiological doses of testosterone undecanoate (TU).Methods: Twenty fertile adult men received four intramuscular injections of TU at monthly intervals, 1000 mg uponadmission and 500 mg for the subsequent injections. The apoptotic germ cells in the semen were studied under light mi-croscope with tenninal deoxynucleotidyl tmnsferase-mediated dUTP-biotin nick end labeling (TUNEL) and Wright-Giem-sa staining methods. Results: After treatment, the sperm density and the number of spermatogenic cells in the semenwere significantly decreased ( P 0.01), while the apoptotic ratios of spermatocytes and spermatids increased significantly( P 0.01) as compared with the pretreatment levels. Apoptosis was found to be augmented in the whole series of castoffspennatogenic cells. Conclusion: Besides its suppressive effect on spermatogenesis through a negative feed-backmechanism, TU enhances apoptosis of spermatogenic cells, which may be an additional mechanism of its anti-spermato-genic activity. ( Asian J Androl 1999 Sep; 1: 155 - 158)