Coptidis Rhizoma Water Extract Stimulates 5'-AMP-Activated Protein Kinase in Rat Skeletal Muscle

作     者：Tatsuro Egawa Rieko Oshima Eriko Kurogi Hiroko Tanabe Satoshi Tsuda Tatsuya Hayashi 

作者机构：Laboratory of Sports and Exercise MedicineGraduate School of Human and Environmental StudiesKyoto University 

出 版 物：《中国天然药物》 (Chin. J. Nat. Med.)

年 卷 期：2011年第9卷第3期

页      面：215-221页

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the research grants from the Japan Society for the Promotion of Science (No.20500576) Japan Vascular Disease Research Foundation 

主　　题：Coptidis Rhizoma Coptis chinensis 5’-AMP-activated protein kinase Skeletal muscle Berberine 

摘      要：AIM:Coptidis Rhizoma(CR),the dried rhizomes of Asian herbs(including Coptis chinensis Franch),has been used to treat diabetes mellitus for thousands of *** explored the possibility that CR acts directly on skeletal muscle,the major organ responsible for glucose homeostasis,and activates 5 -AMP-activated protein kinase(AMPK),a signaling intermediary leading to metabolic enhancement of skeletal ***:Isolated rat epitrochlearis and soleus muscles were incubated in a buffer containing a CR water extract(CE),and activation of AMPK and related events were ***:In response to CE treat-ment,phosphorylation of Thr172 at the catalyticαsubunit of AMPK,an essential step for full kinase activation,increased in both *** of Ser79 of acetyl CoA carboxylase(ACC),an endogenous substrate of AMPK,increased *** of isoform-specific AMPK activity revealed that CE activated both the α1 and α2 isoforms of the catalytic ***,the maximal effect of CE on AMPK phosphorylation was significantly greater than that of berberine(BBR),indicating that the action of CE is not totally ascribed to ***:We propose that CE is an acute activator of AMPK in both fast-and slow-twitch skeletal muscles.