Variations of Single Nucleotide Polymorphism (SNP) of Hordoindoline a Gene (hina) and Its Relationships with Kernel Hardness in 92 North American Barley Varieties (Hordeum vulgare L.)

作     者：CHEN Guo-yue YANG Shi-dong LI Wei-tao LI Wei WEI Yu-ming ZHENG You-liang 

作者机构：Triticeae Research Institute Sichuan Agricultural University Ya'an 625014 P.R.China Key Laboratory of Crop Genetic Resources and Improvement in Southwest China Ministry of Education/Sichuan Agricultural University Ya'an 625014 P.R. China College of Agronomy Sichuan Agricultural University Ya'an 625014 P.R.China 

出 版 物：《Agricultural Sciences in China》 (中国农业科学（英文版）)

年 卷 期：2009年第8卷第12期

页      面：1440-1447页

核心收录：

学科分类：0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 07[理学] 08[工学] 071007[理学-遗传学] 0836[工学-生物工程] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 083203[工学-农产品加工及贮藏工程] 

基　　金：supported by the Key Technologies R&D Program of China (2006BAD01A02-23 and 2006BAD13B02-06) the National High-Tech R&D Program of China (863 Program, 2006AA10Z179 and 2006AA10Z1F8) the Foundation for the Authors of National Excellent Doctoral Dissertation of P.R. China(FANEDD,200357) from Ministry of Education,China 

主　　题：barley Hordoindoline a gene hardness SNP haplotype 

摘      要：Grain texture in barley （Hordeum vulgare L.） is an important quality character. The single nucleotide polymorphisms （SNPs） in Hordoindoline a （hina） gene and its relationship with hardness index among North American harley cultivars were investigated. The hina genes in 36 cultivars were sequenced for the SNP analyses, and 17 sets of SNP primers were designed to detect the SNP variations of hina locus in 92 North American barley cultivars. SNP detection indicated that there were four haplotypes in the hina genes of 92 barley cultivars, and haplotype 01 and 02 were shared by 68 and 14 cultivars, respectively, suggesting that there was a very limited diversity in hina genes among North American barley cultivars. Despite the wide range in hardness exists in 92 barley cultivars, however, unlike wheat, where a clear relationship has been demonstrated between a number of SNPs in the wheat hardness genes and quality （soft or hard wheat）, there was no such relationship for barley. The genotypes used in this study demonstrated that there was a low level of polymorphism in hina gene in North American barley cultivars and these polymorphisms had no impact on grain hardness.