Functional characterization of WRKY46 in grape and its putative role in the interaction between grape and phylloxera (Daktulosphaira vitifoliae)

作     者：Feng-Pan Wang Pan-Pan Zhao Lei Zhang Heng Zhai Yuan-Peng Du 

作者机构：State Key Laboratory of Crop BiologyKey Laboratory of Biology and Genetic Improvement of Horticultural Crops(Huang-Huai RegionMinistry of Agriculture)College of Horticulture Science and EngineeringShandong Agricultural UniversityTai-an 271000 ShandongChina Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong ProvinceCollege of Optoelectronic EngineeringShenzhen UniversityShenzhen 518060 GuangdongChina Guangdong Provincial Key Laboratory for Plant EpigeneticsCollege of Life Sciences and OceanographyShenzhen UniversityShen-zhen 518060 GuangdongChina College of Biological and Enology EngineeringTaishan UniversityTai-an 271000 ShandongChina 

出 版 物：《Horticulture Research》 (园艺研究（英文）)

年 卷 期：2019年第6卷第1期

页      面：504-517页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：supported by the National Natural Science Foundation of China in 2012(31201609) 

主　　题：interaction interaction characterization 

摘      要：WRKY transcription factors are involved in defense responses caused by biotic ***(Daktulosphaira vitifoliae Fitch),a pest widespread in viticulture,elicits transcriptional reprogramming of plant defense-associated components,such as regulons related to WRKYs and salicylic acid(SA)*** this study,we characterized WRKY46,a WRKY transcription factor responsible for phylloxera attack,and revealed the molecular mechanism for WRKY-mediated defense responses to ***-PCR and GUS staining analyses revealed that WRKY46 is induced in response to phylloxera damage and mechanical ***46 is a nuclear-localized transcription factor that activates its downstream target VvCHIB by direct protein–DNA *** involved in the SA-mediated defense response were regulated during incompatible interactions between“1103 Paulsenrootstock and *** addition,WRKY46 exhibited a higher transcript abundance in“1103 Paulsenthan in“Crimson Seedless,regardless of whether the plants were infected with ***,the enhanced expression of VvWRKY46 significantly attenuated phylloxera attack and delayed nymph development of composite grape *** summary,we demonstrated that WRKY46 plays a role in the SA-mediated defense-regulatory network by directly binding to the downstream structural gene *** phylloxera-responsive gene WRKY46 was identified,which could improve the understanding of the basic mechanism of grapevine in response to phylloxera.