Rapid one-step enzyme immunoassay and lateral flow immunochromatographic assay for colistin in animal feed and food

作     者：Jiayi Wang Jinyu Zhou Yiqiang Chen Xinpei Zhang Yongpeng Jin Xiaojing Cui Dongting He Wenqing Lai Lidong He Jiayi Wang;Jinyu Zhou;Yiqiang Chen;Xinpei Zhang;Yongpeng Jin;Xiaojing Cui;Dongting He;Wenqing Lai;Lidong He

作者机构：Beijing Advanced Innovation Center for Food Nutrition and Human Healthand State Key Laboratory of Animal NutritionCollege of Animal Science and TechnologyChina Agricultural UniversityBeijingChina Department of Chemistry and BiochemistryFlorida State UniversityTallahasseeFLUSA. 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2020年第11卷第1期

页      面：280-289页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 081704[工学-应用化学] 07[理学] 0905[农学-畜牧学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：financially supported by Beijing Advanced Innovation Center for Food Nutrition and Human Health Basic Research Program of Science and Technology(2014FY111000) 

主　　题：Colistin ELISA Feed Food Gold nanoparticle Lateral flow immunochromatographic assay Monoclonal antibody 

摘      要：Background:Colistin(polymyxin E)is a kind of peptide antibiotic which has been approved in animal production for the purposes of disease prevention,treatment,and growth ***,the wide use of colistin in animal feed may accelerate the spread of colistin-resistance gene MCR-1 from animal production to human beings,and its residue in animal-origin food may also pose serious health hazards to ***,it is necessary to develop corresponding analytical methods to monitor the addition of colistin in animal feed and the colistin residue in animal-origin ***:A one-step enzyme-linked immunosorbent assay(ELISA)and a lateral flow immunochromatographic assay(LFIA)for colistin were developed based on a newly developed monoclonal *** ELISA showed a 50%inhibition value(IC50)of 9.7 ng/m L with assay time less than 60 min,while the LFIA had a strip reader-based detection limit of 0.87 ng/m L in phosphate buffer with assay time less than 15 *** reducing the non-specific adsorption of colistin onto sample vial,the components of sample extraction solution were optimized and proved to greatly improve the assay *** spiked recovery experiment showed that the recoveries of colistin from feed,milk and meat samples were in the range of 77.83%to 113.38%with coefficient of variations less than 13%by ELISA analysis and less than 18%by LFIA analysis,***,actual sample analysis indicated that the two immunoassays can produce results consistent with instrumental ***:The developed assays can be used for rapid qualitative or quantitative detection of colistin in animal feed and food.