Heat induces adenosine triphosphate release from mast cells in vitro:a putative mechanism for moxibustion

作     者：Hu Lei Wang Lina Wei Jianzi Ryszard Grygorczyk Shen Xueyong Wolfgang Schwarz 

作者机构：Acupuncture & Moxibustion and Tuina CollegeShanghai University of Traditional Chinese Medicine Cellular Electrophysiological LaboratoryShanghai Research Centre for Acupuncture and Meridians Research CentreCentre hospitalierde I'Universite de Montreal(CRCHUM)-Hotel-DieuQuebec H2W 1T8Canada Department of MedicineUniversite de Montreal Institute for BiophysicsGoethe-University Frankfurt 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2015年第35卷第3期

页      面：323-328页

核心收录：

学科分类：1005[医学-中医学] 100512[医学-针灸推拿学] 10[医学] 

基　　金：National Natural Science Foundation of China(P2Y Receptors in Acupoints Involved in the Analgesic Effects of Laser Acupuncture(No.81102635) Efficacy and Neurobiological Mechanisms of Traditional MoxibustionBased Laser Moxibustion with Specific Wavelength on Inflammatory Pain(No.81320108028)) The National Basic Research Program of China(973 programs,Basic Research on Trigger Signals in Acupoints and Specific Modulation of Correlated Target Viscera(No.2012CB518502) Biologic Research on Characteristics of Infrared Physics and Mechanisms in Moxibustion,(No.2015CB554505)) The Project of State Administration of Traditional Chinese Medicine of China(Acupuncture,No.ZYSNXD-CC-ZDXK-07) 

主　　题：Fire toxin syndrome Mast cells Cell degranulation Calcium Adenosine triphosphate Moxibustion 

摘      要：OBJECTIVE:To investigate the role of adenosine triphosphate(ATP) purinergic signaling in mast cells(MCs) modulated by heat to further understand the molecular mechanisms of ***:Skin temperatures induced by monkshood cake moxibustion were evaluated by measuring the Neiguan acupoint(PC 6) from 31 participants with a digital thermocouple *** of 43 ℃ and 52 ℃ were applied to cultured human leukemia mast cell line HMC-1 in *** fluorescence was applied to detect intracellular Ca^(2+)([Ca^(2+)]_i).Extracellular ATP contents were measured by luciferin-luciferase ***:Maximum skin temperatures mostly ranged from 40-45 ℃,but some reached up to50 ℃.Both 43 ℃,and 52 ℃ induced MC degranulation,which was accompanied by an increase in[Ca^(2+)]_i and ATP *** extracellular Ca^(2+) with 5 mM ethylene glycol-bis(β-aminoethyl ether)-N,N,N ,N -tetraacetic acid(EGTA) inhibited the noxious heat-induced elevation of[Ca^(2+)]_i and prevented the enhanced ATP secretion by those cells at 52℃ but not 43 ℃.CONCLUSION:Monkshood cake moxibustion can generate heat sufficient to trigger cellular events of MCs,including degranulation,[Ca^(2+)]_i elevation,and ATP release,suggesting that purinergic signals originating from MCs are possibly the initiating response of acupoints to moxibustion.