Effect of Alstonia scholaris bark extract on testicular function of Wistar rats

作     者：R. S. Gupta Rakhi Sharma Aruna Sharma A.K. Bhatnager M.P. Dobhal Y.C. Joshi M.C. Sharma R. S. GUPTA~;RAKHI SHARMA~;ARUNA SHARMA~;A.K. BHATNAGER~;M.P. DOBHAL~;Y.C. JOSHI~;M.C. SHARMA~ ~REPRODUCTION PHYSIOLOGY SECTION;DEPARTMENT OF ZOOLOGY;UNIVERSITY OF RAJASTHAN;JAIPUR-;INDIA ~DEPARTMENT OF CHEMISTRY;S.S. JAIN SUBODH P.G. COLLEGE;INDIA ~ROSWELL PARK CANCER INSTITUTE;ELM & CARLTON STREETS;BUFFALO;NEW YORK;USA ~DEPARTMENT OF CHEMISTRY;UNIVERSITY OFRAJASTHAN;INDIA

作者机构：Reproduction Physiology Section Department of Zoology University of Rajasthan Jaipur-302004 IndiaReproduction Physiology Section Department of Zoology University of Rajasthan Jaipur-302004 IndiaReproduction Physiology Section Department of Zoology University of Rajasthan Jaipur-302004 IndiaDepartment of Chemistry S.S. Jain Subodh P.G. College Jaipur-302004 IndiaRoswell Park Cancer Institute Elm & Carlton Streets Buffalo New York 14263 USADepartment of Chemistry University of Rajasthan Jaipur-302004 IndiaDepartment of Chemistry University of Rajasthan Jaipur-302004 India 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：2002年第4卷第3期

页      面：175-178页

核心收录：

学科分类：10[医学] 

主　　题：Alstonia scholaris testis sperm motility sialic acid Sertoli cells 

摘      要：Abstract Aim: To evaluate the antifertility effect ofAlstonia scholaris bark extract in male rats. Methods: In male Wistar rats Alstonia scholaris bark extract was given by oral route at a dose of 200 mg/day for 60 days. The fertility and testicular function were assessed by mating tests, sperm motility, sperm concentration, biochemical indices and testicular cell population dynamics. Results: Oral feeding with the extract at a dose of 200 mg/day for the period of 60 days did not cause body weight loss, while the weights of testes, epididymides, seminal vesicle and ventral prostate were significantly reduced. The production of step-19 spermatids was reduced by 79.6% in treated rats. The population of preleptotene and pachytene spermatocytes were decreased by 61.9% and 60.1%, respectively. Spermatogonia and Sertoli cell population were also affected. The seminiferous tubule and Leydig cell nuclear area were reduced significantly (P0.01) when compared to the controls. Reduced sperm count and motility resulted in a total suppression of fertility. A significant fall in the protein and sialic acid content of the testes, epididymides, seminal vesicle and ventral prostate as well as glycogen content of testes were also noticed. The fructose content in the seminal vesicle was lowered whereas the testicular cholesterol was elevated as compared with the controls. The following compounds were obtained from the extract with chromatographic separation over Si-gel column: α-amyrin, β-amyrin, lupiol acetate, venenative, rhazine and yohimbine. Conclusion: At the dose level employed, Alstonia scholaris bark extract has a significant antifertility effect in male rats; the primary site of action may be post meiotic germ cells (Step 19 spermatids).