Isolation and Identification of Four Strains of Salmonella and Drug Susceptibility Test

作     者：Guo Rui Ding Jialin Luo Junfeng Feng Ling Liu Zhiqiang Zhao Xiaocui Yao Weiping Gao Qingshan Fu Yanfang Su Shuoqing Shi Qiumei 

作者机构：Changli Bureau of Agriculture Forestry Animal Husbandry and Aquatic Products Changli 066600 China Qilu Shenghua Pharmaceutical Co. Ltd. Linyi 255399 China Animal Husbandry Bureau of Haigang District Qinhuangdao 066004 China Leting Animal Husbandry Bureau Leting 063600 China Liushouying Animal Epidemic Surveillance Station Agriculture and Animal Husbandry Bureau of Funing District Funing 066300 China Animal Epidemic Prevention Station of Luanzhou Town Luanzhou Bureau of Animal Husbandry and Aquatic Products Luanzhou 063799 China Hebei Animal Husbandry Station Shijiazhuang 050000 China Hebei Normal University of Science and Technology/Hebei Key Laboratory of Preventive Veterinary Medicine Qinhuangdao 066604 China 

出 版 物：《Animal Husbandry and Feed Science》 (动物与饲料科学（英文版）)

年 卷 期：2019年第11卷第1期

页      面：39-41,44页

学科分类：09[农学] 

基　　金：Supported by The Third Batch Giant Plan of Hebei Province(180416H) 

主　　题：Chicken Salmonella Isolation and identification Drug susceptibility test 

摘      要：In order to isolate and identify the pathogenic bacteria causing dead chickens in a chicken farm in Qinhuangdao area, the liver, heart and other organs of dead chickens suspected of salmonella disease were collected aseptically, and streaked on SS agar medium and chromagar medium. Transparent colonies were observed on SS agar medium, and purple and transparent colonies on CAS medium. The isolate was conducted purification, staining microscopy, biochemical tests, and 16 S rRNA sequence analysis, and the results showed that four strains of the isolated bacte-ria were salmonella. The 16 S rRNA sequence analysis of four strains of salmonella showed that the isolates shared more than 99% homology. Drug susceptibility test was performed using paper method, and the results showed that most of the strains were resistant to tilmicosin, cefradine and sul-famethoxazole, but were sensitive to ceftriaxone.