Changes in Anatomical Features and Protein Pattern of Sunflower Partially Resistant and Susceptible Lines During Infection By Virulence Factors of Sclerotinia Sclerotiorum
作者机构：Department of Plant Molecular BiotechnologyInstitute of Agricultural BiotechnologyNational Institute of Genetic Engineering and BiotechnologyTehranPO Box 14925/16Iran Department of Agri-FoodAnimal and Environmental SciencesUniversity of UdineUdineItaly.
出 版 物：《Phyton-International Journal of Experimental Botany》 (国际实验植物学杂志（英文）)
年 卷 期：2019年第88卷第2期
页 面：149-159页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学]
基 金：National Institute for Genetic Engineering and Biotechnology NIGEB (666)
主 题：Microtome Xylem Helianthus annuus Sclerotinia sclerotiorum Stem rot
摘 要：Helianthus annuus *** an oil seed crop is widely grown throughout the *** of the most destructive diseases of sunflower is stem rot caused by Sclerotinia *** acid is the major virulence factor of this necrotrophic *** is important to further investigate plant responses to this non-specific ***,in the present study,we compared the patterns of total soluble proteins and xylem morphology of partially resistant and susceptible sunflower lines after treatment with Sclerotinia culture *** basal stems of both lines were treated with 40 mM oxalic acid(pH 3.7)of fungus culture filtrate and samples were collected at 24,48 and 72 hours post *** SDS-PAGE protein pattern new protein bands appeared in both lines after *** observations suggest induction of stress-related proteins upon culture filtrate *** identities of these new proteins need to be more clarify in future *** changes in xylem morphology and degree of lignification of both lines was studied by light microscopy and microtome sectioning techniques after treatment with *** culture *** investigations revealed changes in xylem diameter and xylem lignification of treated lines at various time *** lignin deposition in xylem vessels of partially resistant line has been observed after *** addition,the size of xylem vessels in partially resistant line has been sharply decreased upon pathogen filtrate *** results of this study will help us gain a more complete understanding of resistance mechanisms to this cosmopolitan and devastating pathogen.
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