Changes in Anatomical Features and Protein Pattern of Sunflower Partially Resistant and Susceptible Lines During Infection By Virulence Factors of Sclerotinia Sclerotiorum
作者机构:Department of Plant Molecular BiotechnologyInstitute of Agricultural BiotechnologyNational Institute of Genetic Engineering and BiotechnologyTehranPO Box 14925/16Iran Department of Agri-FoodAnimal and Environmental SciencesUniversity of UdineUdineItaly.
出 版 物:《Phyton-International Journal of Experimental Botany》 (国际实验植物学杂志(英文))
年 卷 期:2019年第88卷第2期
页 面:149-159页
核心收录:
学科分类:0710[理学-生物学] 071001[理学-植物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学]
基 金:National Institute for Genetic Engineering and Biotechnology NIGEB (666)
主 题:Microtome Xylem Helianthus annuus Sclerotinia sclerotiorum Stem rot
摘 要:Helianthus annuus *** an oil seed crop is widely grown throughout the *** of the most destructive diseases of sunflower is stem rot caused by Sclerotinia *** acid is the major virulence factor of this necrotrophic *** is important to further investigate plant responses to this non-specific ***,in the present study,we compared the patterns of total soluble proteins and xylem morphology of partially resistant and susceptible sunflower lines after treatment with Sclerotinia culture *** basal stems of both lines were treated with 40 mM oxalic acid(pH 3.7)of fungus culture filtrate and samples were collected at 24,48 and 72 hours post *** SDS-PAGE protein pattern new protein bands appeared in both lines after *** observations suggest induction of stress-related proteins upon culture filtrate *** identities of these new proteins need to be more clarify in future *** changes in xylem morphology and degree of lignification of both lines was studied by light microscopy and microtome sectioning techniques after treatment with *** culture *** investigations revealed changes in xylem diameter and xylem lignification of treated lines at various time *** lignin deposition in xylem vessels of partially resistant line has been observed after *** addition,the size of xylem vessels in partially resistant line has been sharply decreased upon pathogen filtrate *** results of this study will help us gain a more complete understanding of resistance mechanisms to this cosmopolitan and devastating pathogen.