Nanotechnology based CRISPR/Cas9 system delivery for genome editing:Progress and prospect

作     者：Huan Deng Wei Huang Zhiping Zhang 

作者机构：Tongji School of PharmacyTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of OrthopedicsUnion HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China National Engineering Research Center for NanomedicineHuazhong University of Science and TechnologyWuhan 430030China Hubei Engineering Research Center for Novel Drug Delivery SystemHuazhong University of Science and TechnologyWuhan 430030China 

出 版 物：《Nano Research》 (纳米研究（英文版）)

年 卷 期：2019年第12卷第10期

页      面：2437-2450页

核心收录：

学科分类：0710[理学-生物学] 0808[工学-电气工程] 0809[工学-电子科学与技术（可授工学、理学学位）] 07[理学] 08[工学] 09[农学] 0805[工学-材料科学与工程（可授工学、理学学位）] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 0702[理学-物理学] 

基　　金：This work was supported by the National Natural Science Foundation of China(Nos.81673374 and 81872810) Wuhan Science and Technology Plan for Applied Fundamental Research(No.2017060201010146) Fundamental Research Funds for the Central Universities(No.2018KFYYXJJ019) 

主　　题：clustered regularly in terspaced short palindromic repeats(CRISPR)/Cas9 genome editi ng nan oparticle polymer liposome 

摘      要：The genome editing tool,clustered regularly interspaced short palindromic repeats(CRISPR)/Cas9 system,has achieved successful therapeutic efficacy via precise modification of the genome and exceeded previous genome engineering methods owing to its versatility and *** expansion in biomedical research has benefited from this newly emerged technique,such as genetic diseases treatment,cancer characterization,and plant ***,the key challenge is efficient delivery of CRISPR components in vivo and nanotechnology plays an in dispensable role in non viral gene *** this review,we will first briefly describe the mechanism and delivery strategies of CRISPR/Cas9 ***,the past and current researches of nan oparticles based CRISPR/Cas9 system delivery for genome editi ng will be *** ally,we will discuss the challe nges and prospects of CRISPR/Cas9 system combi ned with nano tech no logy for clinical translation in the future.