Implementation and application of FRET-FLIM technology
作者机构：Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen UniversityShenzhen518060P.R.China
出 版 物：《Journal of Innovative Optical Health Sciences》 (创新光学健康科学杂志（英文）)
年 卷 期：2019年第12卷第5期
页 面：57-68页
核心收录：
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学]
基 金：supported by the National Natural Science Foundation of China(61722508/61525503/61620106016/81727804) The National Key Research and Development Program of China(2017YFA0700402) Guangdong Natural Science Foundation Innovation Team(2014A030312008) Shenzhen Basic Research Project(JCYJ20150930104948169/JCYJ20160328144746940/GJHZ20160226202139185/JCYJ20170412105003520)
主 题：Fluorescence resonance energy transfer fuorescenc-lifetime imaging microscopy protein-protein interaction
摘 要：With the development of the new detection methods and the function of fluorescent molecule,researchers hope to further explore the internal mechanisms of organisms,monitor changes in the intracellular microenvironment,and dynamic processes of molecular interactions in ***-rescence resonance energy transfer(FRET)describes the energy transfer process between donor fluorescent molecules and acceptor fluorescent *** is an important means to detect protein-protein interactions and protein conformation changes in *** lifetime imaging microscopy(FLIM)enables noninvasive measurement of the fAuorescence lifetime of fluorescent particles in *** FRET-FLIM technology,which is use FLIM to quantify and analyze FRET,enables real-time monitoring of dynamic changes of proteins in biological cells and analysis of protein interaction *** distance between donor and acceptor and their respective fAuorescent lifetime,which are of great importance for studying the mechanism of intracellular activity can be obtained by data analysis and algorithm ftting.
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