Cytotoxicity of Quartz and Montomrillointe in Human Lung Epthlial Cell

作     者：HUO Tingting DONG Faqin WANG Mingcui SUN Shiyong DENG Jianjun ZHANG Qingbi YU Siwang 

作者机构：School of Environmental and ResourceSWUST Key Laboratory of Solid Waste Treatment and Resource RecycleSWUSTMinistry of Education The Mianyang 404 Hospital Luzhou Medical College Department of Chemical BiologySchool of Pharmaceutical ScoencePecking University Health Science Center 

出 版 物：《矿物学报》 (Acta Mineralogica Sinica)

年 卷 期：2013年第33卷第S1期

页      面：37-37页

核心收录：

学科分类：100405[医学-卫生毒理学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0709[理学-地质学] 070901[理学-矿物学、岩石学、矿床学] 07[理学] 10[医学] 

主　　题：quartz montmorillonite A549 cytotoxicity genotoxicity 

摘      要：In this study A549 cell viability, extracellular activities of lactate dehydrogenase (LDH), tumor necrosis factor (TNF)–α and interleukin (IL)-6 levels were investgated after incubation with quartz (KWC-Q4 and KWC-Q3), Nano-SiO2, and KWC-M, the micronucleaus test and comet assay was carried out to evaluate the genotoxicity. The results showed that, there were significant difference in the cell death rate and extracellular LDH activity compared with the control group, and appeared a good linear relationship in certain concentration range. All mineral particle tested can induce the increase of TNF-α after incubation with mineral powders at 200 μg/mL for 3h merely, and significant increase of IL-6 for 24h, the results indicated the inflammatory reaction can be triggered by the exposure of KWC-Q4, KWC-Q3, Nano-SiO2, and KWC-M. The micronucleus test result showed the MNF (Micronucleus frequency) listed as Nano-SiO2KWC-Q3KWC-Q4. There is no significant increase of KWC-M compared with the control group, maybe resulted from its high cell death rate at low concentration. The comet assay confirmed the genotoxicity of all samples tested, the DNA damage: KWC-M Nano-SiO2 KWC-Q4 KWC-Q3.