The Concurrence of Hypercholesterolemia and Aging Promotes DNA Damage in Apolipoprotein E-Deficient Mice
The Concurrence of Hypercholesterolemia and Aging Promotes DNA Damage in Apolipoprotein E-Deficient Mice作者机构：Biotechnology Graduate Program Health Sciences Center Federal University of Espirito Santo Vitoria ES Brazil Biotechnology Graduate Program Health Sciences Center Federal University of Espirito Santo Vitoria ES Brazil Dept. Physiological Sciences Health Sciences Center Federal University of Espirito Santo Vitoria ES Brazil Dept. Physiological Sciences Health Sciences Center Federal University of Espirito Santo Vitoria ES Brazil Emescam College of Health Sciences ES Brazil
出 版 物：《Open Journal of Blood Diseases》 (血液病期刊（英文）)
年 卷 期：2012年第2卷第3期
页 面：51-55页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：DNA Damage Oxidative Stress apoE Knockout Mice Aging Blood Cells
摘 要：Recent evidence shows that increased oxidative stress and aging contribute to DNA damage in various cardiovascular diseases such as lipid disorders and atherosclerosis. In the present study, we used the comet assay to evaluate the influ- ence of aging on DNA damage in whole blood cells from apolipoprotein E-deficient (apoE?/?) mice and compared the results to those found in cells from wild-type C57BL/6 (C57) mice. Using the alkaline comet assay and fluorescent ethidium bromide staining, DNA damage was analyzed in the peripheral whole blood (5 μL) cells that were isolated from either young (8-week-old) and elderly (72-week-old) apoE?/? mice or from age-matched C57 mice. The levels of total plasma cholesterol were approximately 6-fold higher in apoE?/? mice of both ages compared to C57 mice. Elderly apoE?/? mice showed significantly higher levels of DNA damage (19%) compared to elderly C57 mice (8%, p 0.01) and young apoE?/? mice (10%, p 0.01). The comet assay in whole blood cells is a suitable technique for the detection of DNA damage in the apoE?/? mouse;it is an easy, rapid, inexpensive and sensitive method. The novelty of this study is that DNA damage occurring in whole blood cells of this murine model requires the concurrence of aging and oxida- tive stress-related hypercholesterolemia.
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