Calibration of CO<sub>2</sub>Trapping in Alkaline Solutions during Soil Incubation at Varying Temperatures Using a Respicond VI
Calibration of CO<sub>2</sub>Trapping in Alkaline Solutions during Soil Incubation at Varying Temperatures Using a Respicond VI作者机构：Institute of Crop Science (340i) University of Hohenheim Stuttgart Germany Institute of Plant Production and Agroecology in the Tropics and Subtropics (380a) University of Hohenheim Stuttgart Germany
出 版 物：《Open Journal of Soil Science》 (土壤科学期刊（英文）)
年 卷 期：2014年第4卷第5期
页 面：161-167页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Respirometer Soil Respiration Carbon Dioxide Incubation
摘 要：Measurements of carbon dioxide (CO2)-evolution from soils are important in evaluating biomass and activity of soil microorganisms, as well as decomposition of soil organic matter. The Respicond VI is a fully computerized system allowing continuous measurement of CO2 evolution in short- and long-term soil incubation experiments in up to 96 incubation vessels. The measurement of CO2 evolution is based on the absorption of CO2 by an electrolyte (KOH solution) producing a change in the cell conductance measured using two electrodes. In this study, the Respicond VI was recalibrated yielding 174.5 mg CO2 as constant A expressing the theoretical maximum amount of CO2 absorbed in 10 ml 0.5 M KOH. This value of A corresponds to 34.9 mg CO2 ml-1 1 M KOH. The constant A does neither depend on the investigated incubation temperatures (5°C - 25°C) nor on the concentrations of the KOH solutions (0.5, 0.1, 0.05 M KOH). To eliminate any influence of changing incubation temperatures, either induced by uncertainties in temperature control or as a part of the experimental setup, on the conductance of KOH solution, a correction procedure was developed using a factor calculated from changing conductance of KOH solutions in incubation vessels without soil.
维普期刊数据库