Localization of proteins involved in endocytosis at tubulobulbar complexes in rat testes
Localization of proteins involved in endocytosis at tubulobulbar complexes in rat testes作者机构：Department of Neuroendocrinology National Institute for Research in Reproductive Health (ICMR) Mumbai India
出 版 物：《Advances in Reproductive Sciences》 (生殖科学（英文）)
年 卷 期：2014年第2卷第1期
页 面：1-7页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Tubulobulbar Complex Endocytosis Sertoli-Cell Spermiation Junction
摘 要：Tubulobulbar Complexes (TBCs) are actin-rich structures formed between Sertoli-cells and spermatids at the time of sperm release. The main functions of the TBCs are to remove excess spermatid cytoplasm and acrosomal contents, internalize and recycle junctional complexes by endocytosis prior to spermiation. However, in addition to recycling some of the molecules undergo lysosomal degradation. The molecular machinery involved in endocytosis at the TBCs is not well understood. To bridge this gap localization of various proteins, involved at various steps of endocytosis studied in other systems, was demonstrated in TBCs using testicular fragmented material or sections by immunoblotting and immunofluroscence. The presence of key molecules like Vamp-2, syntaxin and Lamp-2 indicates occurrence of lysosomal degradation in addition to junctional recycling at the TBCs present at the time of sperm release. TBCs are endocytic devices functioning to recycle junctional molecules or remove spermatid cytoplasm that were present between spermatids and Sertoli-cells all through the process of spermatid maturation and in turn regulate male fertility.
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