Investigation of the Anti-Mycobacterial Mechanism of Action of 7-Methyljuglone

作     者：Veneesha Thaver J. J. M. Meyer Riana Cockeran Moloko C. Cholo Ronald Anderson Namrita Lall 

作者机构：Department of Physiology University of Limpopo Medical University of South Africa Campus Pretoria South Africa Department of Plant Science Faculty of Natural and Agricultural Sciences University of Pretoria Pretoria South Africa MRC Unit for Inflammation and Immunity Department of Immunology Faculty of Health Sciences University of Pretoria and Tshwane Academic Division of the National Health Laboratory Service Pretoria South Africa 

出 版 物：《Open Journal of Respiratory Diseases》 (呼吸病期刊（英文）)

年 卷 期：2012年第2卷第2期

页      面：60-62页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Adenosine Triphosphate Cell Membrane Energy Metabolism Potassium 

摘      要：Objectives: Although the naphthoquinone, 7-methyljuglone (7-MJ), is active against Mycobacterium tuberculosis (MTB) in vitro, neither the cellular site nor mechanism of anti-mycobacterial action of this agent has been identified. The primary objective of the current study was to investigate the mycobacterial outer membrane as a potential target of 7-MJ by measuring the effects of this agent (0.023 - 1.5 mg/L) on microbial ATP levels and uptake of K+ . Methods: Bioluminescence and radiometric (uptake of 86Rb+) procedures were used to assay microbial ATP levels and K+ transport respectively. Results: Exposure of MTB (strain H37Rv) to 7-MJ for 60 min resulted in dose-related decreases in both microbial ATP levels and uptake of 86Rb+ which achieved statistical significance (P + transport.