Apoptosis of A549 cells by small interfering RNA targeting survivin delivery using poly-β-amino ester/guanidinylated O-carboxymethyl chitosan nanoparticles

作     者：Yue Tang Yun Liu Yuwen Xie Jing Chen Yushun Dou Yue Tang;Yun Liu;Yuwen Xie;Jing Chen;Yushun Dou

作者机构：Department of PharmacyChina Pharmaceutical UniversityNanjing 211198China 

出 版 物：《Asian Journal of Pharmaceutical Sciences》 (亚洲药物制剂科学（英文）)

年 卷 期：2020年第15卷第1期

页      面：121-128页

核心收录：

学科分类：100702[医学-药剂学] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：This work was supported by the 3rd Jiangsu Overseas Research&Training Program for University Prominent Young&Middleaged Teachers and Presidents the College Students Innovation Project for the R&D of Novel Drugs[No.J1310032] And we would like to thank cell and molecular biology experiment platform of China Pharmaceutical University for the assistance with relevant test items 

主　　题：Poly-β-amino ester Guanidinylated O-carboxymethyl chitosan Nanoparticles Gene delivery 

摘      要：Gene-based therapeutics has emerged as a promising approach for human cancer therapy. Among a variety of non-viral vectors, polymer vectors are particularly attractive due to their safety and multivalent groups on their surface. This study focuses on guanidinylated O-carboxymethyl chitosan(GOCMCS) along with poly-β-amino ester(PBAE) for si RNA delivery. Binding efficiency of PBAE/si RNA/GOCMCS nanoparticles were characterized by gel electrophoresis. The si RNA-loaded nanoparticles were found to be stable in the presence of RNase A, serum and BALF respectively. Fine particle fraction(FPF) which was determined by a two-stage impinger(TSI) was 57.8% ± 2.6%. The particle size and zeta potential of the nanoparticles were 153.8 ± 12.54 nm and + 12.2 ± 4.94 m V. In vitro cell transfection studies were carried out with A549 cells. The cellular uptake was significantly increased. When the cells were incubated with si Survivin-loaded nanoparticles, it could induce 26.83% ± 0.59% apoptosis of A549 cells and the gene silencing level of survivin expression in A549 cells were 30.93% ± 2.27%. The results suggested that PBAE/GOCMCS nanoparticle was a very promising gene delivery carrier.