Macrophage-derived matrix metalloproteinase-1 enhances aortic aneurysm formation in transgenic rabbits

作     者：Manabu Niimi Kazutoshi Nishijima Shuji Kitajima Fumikazu Matsuhisa Kaneo Satoh Hirokazu Yamazaki Jifeng Zhang Y. Eugene Chen Jianglin Fan 

作者机构：Department of Molecular PathologyGraduate School of MedicineUniversity of YamanashiYamanashi 409-3898Japan Animal Research LaboratoryBioscience Education-Research Support CenterAkita UniversityAkita 010-8502Japan Analytical Research Center for Experimental SciencesSaga UniversitySaga 840-8502Japan Department of Laboratory MedicineGraduate School of MedicineUniversity of YamanashiYamanashi 409-3898Japan Center for Advanced Models for Translational Sciences and TherapeuticsUniversity of Michigan Medical CenterAnn ArborMI 48109USA School of Biotechnology and Health SciencesWuyi UniversityJiangmenGuangdong 529020China 

出 版 物：《The Journal of Biomedical Research》 (生物医学研究杂志（英文版）)

年 卷 期：2019年第33卷第4期

页      面：271-279页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported in part by research grants from JSPS KAKENHI(JP26460486 to MN and JP15H04718 to JF) NIH grants(R01HL117491and RO1HL129778 to YEC) 

主　　题：matrix metalloproteinase-1 macrophage atherosclerosis aneurysm transgenic rabbit 

摘      要：Increased expression of matrix metalloproteinase-1(MMP-1)has been observed in the lesions of atherosclerosis and aneurysms;however,it is not fully understood whether macrophage-derived MMP-1 affects these *** investigate whether macrophage-derived MMP-1 participates in the development of vascular diseases,we generated transgenic(Tg)rabbits expressing human MMP-1 in the monocyte/macrophage lineage under the control of the human scavenger receptor enhancer/*** rabbits exhibited no visible abnormalities throughout their *** blotting analysis revealed that the amount of MMP-1 proteins in the conditioned media secreted from peritoneal macrophages of Tg rabbits was up to 3-fold higher than that in non-Tg *** the first experiment,Tg and non-Tg rabbits were fed a cholesterol diet for 16 weeks,and aortic and coronary atherosclerosis were *** gross lesion area of aortic atherosclerosis in Tg rabbits was not significantly different from that in non-Tg rabbits,but Tg rabbits had marked destruction of the medial elastic lamina of the aortic lesions on microscopic *** the second experiment,we generated aortic aneurysms by incubating with *** with non-Tg rabbits,Tg rabbits exhibited a significantly greater aortic *** macrophage-derived MMP-1 led to increased medial destruction in both aortic atherosclerosis and *** results demonstrate that MMP-1 plays a different role in the pathogenesis of atherosclerosis and aneurysms.