The Conserved Proline Residue in the LOB Domain of LBD18 Is Critical for DNA-Binding and Biological Function

作     者：Han Woo Lee Min-Jung Kimb MoungYeon Park Kyung-Hwan Han Jungmook Kim 

作者机构：Department of Bioenergy Science and Technology Chonnam National University Gwangju 500-757 Korea Department of Plant Biotechnology Chonnam National University Gwangju 500-757 Korea Department of Horticulture and Department of Forestry Michigan State University East Lansing MI 48824-1222 USA Kumho Life Science Laboratory Chonnam National University Buk-Gu Gwangju 500-757 Korea 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2013年第6卷第5期

页      面：1722-1725页

核心收录：

学科分类：0710[理学-生物学] 12[管理学] 1201[管理学-管理科学与工程(可授管理学、工学学位)] 09[农学] 0903[农学-农业资源与环境] 

基　　金：supported by grants from the World Class University project of the Ministry of Science, Education Technology of Korea the Next-Generation BioGreen 21 Program Rural Development Administration 

主　　题：LOB 生物学功能 DNA结合 保守 脯氨酸 亮氨酸拉链 残基 器官发育 

摘      要：Dear Editor, The LATERAL ORGAN BOUNDARIES DOMAIN （LBD）/ ASYMMETRICLEAVES2-LIKE （ASL） genes （hereafter referred to as LBD） encode proteins containing a conserved plant-specific LOB domain and play roles in lateral organ development （Iwakawa et al., 2002; Shuai et al., 2002; Majer and Hochholdinger, 2011）. The LOB domain is approximately 100 amino acids in length and contains a conserved four-Cys motif （CX2CX6CX3C）, the Gly-Ala-Ser block （GAS）, and the leucine-zipper-like coiled-coil motif （LX6LX3LX6L） （Shuai et al., 2002）. The leucine-zipper-like coiled-coil motif in the LOB domain is predicted to function in protein dimerization. LOB, AS2, and LBD4 preferentially bind unique DNA sequences in electrophoretic mobility shift assays （EMSAs） （Husbands et al., 2007）. The LOB domain of AS2 cannot be functionally replaced by those of other members of the LOB family,