Role of survivin in apoptosis induced by grape seed procyanidin extract in human bladder cancer BIU87 cells

作     者：Jie Liu Qianyuan Zhuang Weiyi Zhang Yonghua Wang Xianguo Chen Ejun Peng Fei Liu 

作者机构：Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and TechnologyWuhan 430030 China Institute of Integrated Traditional Chinese Medicine and Western Medicine Tongji Hospital Tongji Medical CollegeHuazhong University of Science and Technology Wuhan 430030 China 

出 版 物：《The Chinese-German Journal of Clinical Oncology》 (中德临床肿瘤学杂志（英文版）)

年 卷 期：2009年第8卷第7期

页      面：420-425页

学科分类：0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 07[理学] 08[工学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 083201[工学-食品科学] 090102[农学-作物遗传育种] 

主　　题：apoptosis bladder cancer grape seed procyanidin extract (GSPE) survivin 

摘      要：Objects： The aim of this study was to research the effect of grape seed procyanidin extract （GSPE） on cell apotosis in human bladder cancer BIU87 cells and investigate its molecular mechanism. Methods： BIU87 cells were treated with different concentrations of GSPE and cultured for 24 h in vitro while untreated group as control, MTT[3- （4,5-dimethylthiazole- 2-yl） -2, 5-diphenyltetrazolium bromide] assay, Hoechst 33258 stainning, flow cytometry, RT-PCR and Western blot were used to detect the apoptotic induction effect of GSPE on BIU87 cells. Results： We found that GSPE induced cell apoptosis in BIU87 cells by a dose-dependent manner. Semiquantitated RT-PCR and Western blot analyses indicated that GSPE increased the expression of caspase-3 and decreased the expression of survivin （P 〈 0.01）. Conclusion： GSPE induces apoptosis in BIU87 cells in vitro, and the effect maybe related with its down-regulation of survivin.