Root and shoot traits responses to phosphorus deficiency and QTL analysis at seedling stage using introgression lines of rice

作     者：Junzhou Li Yan Xie Anyong Dai Lifeng Liu Zichao Li 

作者机构：Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture and Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University Beijing 100193 China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2009年第36卷第3期

页      面：173-183页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 09[农学] 0901[农学-作物学] 

基　　金：supported by the Hi-Tech Research and Development Program of China (No. 2006AA10Z158 and No. 2006AA100101) the "948" Project (No. 2006-G1) China National Key Technologies R & D Program (No. 2006BAD13B01-6) the Program for Chang Jiang Scholars and Innovative Research Team in University, Program of Introducing Talents of Discipline to Universi-ties in China (111-2-03). 

主　　题：introgression lines phosphorous deficiency tolerance QTL mapping rice root traits 

摘      要：Phosphorous （P） deficiency is a major restraint factor for crop production and plants have developed several mechanisms to adapt to low P stress. In this study, a set of 271 introgression lines （ILs） were used to characterize the responses of seedlings to low P availability and to identify QTLs for root traits, biomass, and plant height under P-deficiency and P-sufficiency conditions. Plant height, total dry weight, shoot dry weight, and root number were inhibited under P-deficiency, whereas maximum root length （MRL） and root-shoot ratio （RS） were induced by P-deficiency stress. Relative MRL （RMRL, the ratio of MRL under P-deficiency to MRL under P-sufficiency con- dition） and relative RS （RRS） were used to evaluate P-deficiency tolerance at the seedling stage. A total of 24 additive QTLs and 29 pairs of epistatic QTLs were detected, but only qRN4 was detected in both conditions. This suggested that different mechanisms may exist in both P supply levels. QTLs for adaptive traits （RMRL, RRS, RRV, and RRDW） and qRN4 consistently expressed to increase trait stability may contribute to P-deficiency tolerance. Twelve intervals were cluster regions of QTLs for P-deficiency tolerance, and one QTL （qRRSS） showed pleiotropic effects on P-deficiency tolerance and drought tolerance. These interesting QTLs can be used in marker-assisted breeding through the target ILs.