Growth inhibition and induction of apoptosis in human oral squamous cell carcinoma Tca-8113 cell lines by Shikonin was partly through the inactivation of NF-KB pathway

作     者：Min Ruan Tong Ji Wen Hu Duan Wan Tao Chen Chen Ping Zhang 

作者机构：Department of Oral and Maxillofacial SurgeryNinth People's Hospital College of Stomatology Shanghai Jiao Tong University State Key Laboratory of Drug Research Shanghai Institute of Materia Medica SIBS Chinese Academy of ScienceShanghai 200030 China 

出 版 物：《中国口腔颌面外科杂志》 (China Journal of Oral and Maxillofacial Surgery)

年 卷 期：2008年第6卷第B5期

页      面：174-175页

学科分类：1003[医学-口腔医学] 1002[医学-临床医学] 100301[医学-口腔基础医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：口腔 鳞状细胞癌 治疗方法 临床分析 

摘      要：Purpose: To investigate the effects of Shikonin on the proliferation and apoptosis of human oral squamous cell carcinoma Tca-8113 cell line in vitro and the role of NF-kB signal tmnsduction pathway involved。Methods: Inhibitory and apoptosis-induced effect of Shikonin was determined by MTT assay, electron microscopes, the DNA ladder and Flow cytometric analysis。Expression of IK Ba, phosphatase-IK Ba, Bcl-2 and Bax proteins were detected by Westem blot, NF-K B DNA-binding activity was detected by EMSA, and activities of caspase 3, caspase 8 and caspase 9 were analyzed by ELISA。Results: Treatment of the Tca-8113 cells with a variety of concentrations of shikonin (l 0 - 40 la M) resulted in dose- and time-dependent sequences of events marked by apoptosis, as shown by loss of cell viability, chromatin condensation, intemucleosomal DNA fragmentation, and sub-Gl phase accumulation。Furthermore, apoptosis in the Tca-8113 cells was accompanied by the activation ofprotease caspase 8, 9, 3 and low expression ofbcl-2 protein。Interestingly, inactivation of the NF-K B pathway was found in shikonin-induced apoptosis in Tca-8113 cells。Conclusion: Shikonin could effectively inhibit the proliferation of Tca-8113 cell lines and induce apoptosis。Anti-tumor effects of shikonin in Tca-8113 cells act at least partly through the inactivation of NF-K B pathway and subsequent activation of protease caspase family。Pharmacologic inhibition of the NF-K B activity by shikonin might be a powerful treatment option for OSCC。