磁性聚乙烯醇微球固定化α-乙酰乳酸脱羧酶

作     者：钱斯日古楞 王红英 刘阳 耿金峰 朱婧莹 崔茂欣 QIAN Siriguleng;WANG Hongying;LIU Yang;GENG Jinfeng;ZHU Jingying;CUI Maoxin

作者机构：大连工业大学生物与食品工程学院辽宁大连116034 

出 版 物：《化工学报》 (CIESC Journal)

年 卷 期：2007年第58卷第8期

页      面：2062-2066页

核心收录：

学科分类：0817[工学-化学工程与技术] 08[工学] 0703[理学-化学] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 

基　　金：大连市科技攻关项目(2004B3SF175)~~ 

主　　题：磁性微球 聚乙烯醇 α-乙酰乳酸脱羧酶 固定化酶 

摘      要：Magnetic polyvinyl alcohol (PVA) microspheres with 8—64 μm in diameter were prepared from PVA by dispersion and copolymerization with Fe3O4 as *** were more functional groups, such as hydroxyl and carboxyl etc. on the microsphere surface.1,1′-Carbonyldiimidazole (CDI), a carbonylating agent, was used for the activation of hydroxyl groups of PVA, and α-acetolactatedecarboxylase (ALDC) was immobilized onto the magnetic PVA microspheres by covalent bonding through the amino *** results showed that total activity, protein binding, specific activity and activity retention of the immobilized enzyme were 63293 U·g -1, 72.67 mg·g -1, 870.96 U·mg -1 and 48.71%,*** optimum temperature of immobilization enzyme was 50℃ and the optimum pH was *** with the free enzyme of ALDC, the thermal, operational and pH stability of the immobilized enzyme were *** being stored at 4℃, pH 6.0 for 31 days, the immobilized ALDC retained 95.7% of its initial activity which was 8% higher than the free enzyme.