Characterization of Genes Associated with Different Phenotypes of Human Bladder Cancer Cells
Characterization of Genes Associated with Different Phenotypes of Human Bladder Cancer Cells作者机构：Center for Experimental Medicine the First Affiliated Hospital School of Medicine Xi'an Jiaotong UniversityXi'an 710061 China
出 版 物：《Acta Biochimica et Biophysica Sinica》 (生物化学与生物物理学报(英文版))
年 卷 期：2006年第38卷第9期
页 面：602-610页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：bladder cancer phenotypes suppression subtractive hybridization
摘 要：To identify genes associated with morphological phenotypes of human bladder transitional cellcarcinoma,we used suppression subtractive hybridization (SSH) to create a subtractive cDNA library of twoestablished cell lines,BLZ-211 and BLS-211,derived from a patient with transitional cell carcinoma of thebladder,then to screen for differentially expressed ***-time reverse transcription-polymerase chainreaction was used to further confirm the selected differentially expressed *** and reverse subtractivecDNA libraries yielded 168 and 305 putative clones,and among them more than 90% contained the *** differential screening,36 different transcripts were obtained from 64 cDNA clones of a forward andreverse subtraction *** them,17 were identified as known genes by homology,for example,Vimentin,Keratin 7,DDH and *** remaining 19 were unknown expressed genes,and were collectedas new expressed sequence tags by the GenBank dbEST database with the accession numbers DR008207,DR010178,DR159652-DR159660,DY230447-DY230448,and *** function willbe studied ***,SSH appears to be a useful technique for identifying differentially expressed genesbetween cell lines or *** results,as revealed by SSH,also suggest that differences in gene expressionof cytoskeletal proteins might contribute to the different morphologies in BLZ-211 and BLS-211 cells.
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