Isolation of osRACD gene encoding a small GTP-binding protein from rice

作     者：Ml Zhiyong WANG Shusheng WU Naihu 

作者机构：Institute of Developmental BiologyChinese Academy of SciencesBeijing 100080China Academy of Life SciencesPeking UniversityBeijing 100871China 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2002年第47卷第20期

页      面：1673-1679页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：This work was supported by the Fund of the State "863" and the National Basic Sciences Initiative (Grant No. 2001CB1088) 

主　　题：rice osRACD mRNA differential display light signal transduction. 

摘      要：Using an improved version of mRNA differential display technology, we have obtained a differentially displayed fragment RDP-8. Homologous comparison indicated that the fragment RDP-8 has high homology with the gene encoding maize small GTP-binding protein. By screening cDNA library of the rice Nongken 58N panicle using the newly obtained fragment RDP-8 as probe, we further found the full-length cDNA of osRACD gene that encodes a rice small GTP-binding protein. As compared with maize RACD gene, the osRACD of rice shows remarkable homology in both nucleotide sequence and amino acid sequence, 88% and 97% respectively. Evidence from RT-PCR study indicates that osRACD gene is related to photoperiod fertility conversion of photoperiod sensitive genie male sterility (PSGMS) rice.