Effect of hydrogen peroxide on persistent sodium current in guinea pig ventricular myocytes

作     者：Ji-hua MA~2 An-tao LUO Pei-hua ZHANG Cardio-Electrophysiological Research Laboratory,Medical College,Wuhan University of Science and Technology,Wuhan 430080,China 

作者机构：Cardio-Electrophysiological Research Laboratory Medical College Wuhan University of Science and Technology Wuhan China 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2005年第26卷第7期

页      面：828-834页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：the Natural Science Foundation of Hubei Province(No 2003ABA189) 

主　　题：hydrogen peroxide myocardium patch clamp techniques sodium channels 

摘      要：Aim:To study the effect of hydrogen peroxide(H202)on persistent sodium cur-rent(1Na.p)in guinea pig ventricular ***:The whole-cell,cell-attached,and inside-out patch-clamp techniques were applied on isolated ven-tricular myocytes from guinea ***:H2O2(0.1 mmol/L,0.5 mmol/L and 1.0mmol/L)increased the amplitude of whole-cell *** a concentration-dependentmanner,and glutathione(GSH 1 mmol/L)reversed the increased INa.p.H2O2(1mmol/L)increased persistent sodium channel activity in cell-attached and inside-out *** mean open probability was increased from control values of0.015±0.004 and 0.012±0.003 to 0.106±0.011 and 0.136e0.010,respectively(P0.01 vs control).They were then decreased to 0.039±0.024 and 0.027±0.006,respectively,after the addition of 1 mmol/L GSH(P0.01 vs H2O2).The time whenopen probability began to increase and reached a maximum was shorter in inside-out patches than that in cell-attached patches(4.8e 1.0 min vs 11.5+3.9 min,P0.01;9.6±1.6 min vs 18.7±4.7 min,P0.01).Conclusion:H2O2increased the *** pig ventricular myocytes in a concentration-dependent manner,possiblyby directly oxidating the cell membrane.