Moleucle action mechunisms of NM-3 on human gastric cancer SGC-7901 Cells in vivo or in vitro

作     者：Jin-Shui Zhu Bo Shen Jin-Lian Chen Guo-Qiang Chen Xiao-Hu Yu Hua-Fang Yu Zu-Ming Zhu, Affiliated Sixth People’s Hospital, Shanghai Jiaotong University, Shanghai 200233, China Guo-Qiang Chen, Shanghai Experimental Animal Center, Chinese Academy of Sciences, Shanghai 200233, China 

作者机构：Shanghai Jiao Tong Univ Affiliated Peoples Hosp 6 Dept Gastroenterol Shanghai 200233 Peoples R China Chinese Acad Sci Shanghai Expt Anim Ctr Shanghai 200233 Peoples R China 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2003年第9卷第10期

页      面：2366-2369页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Shanghai Natural Science Foundation No.02ZB14072 

主　　题：胃癌 分子机制 SGC-7901细胞 微血管形成 TUNEL法 细胞凋亡 NM-3 

摘      要：AIM: To study the moleucle action mechunisms of NM-3 on the growth of human gastric cancer SGC-7901 cells in vivo or in ***: SGC-7901 from human non-differentiated gastric cancer cell line was cultured with NM-3 at 100 mg/ml for 24 h. We observed its inhibitory rate and the density of micro-vascular growth in grafted mice with human gastric cancer SGC-7901. The apoptosis of human gastric cancer SGC-7901 was revealed in NM-3 treatment group by using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-fluorescene nick end labeling (TUNEL)method and flow cytometry ***: The growth of SGC-7901 cells was markedly inhibited compared with control growp, which was smaller than that in normal saline control group (4.17 g±0.22 g VS 9.45 g±1.38 g, P0.01). The level of apoptosis of human gastric cell line SGC-7901 was obviously increased in NM-3treatment group at 1 mg.L-1 for 24 h. NM-3 inducing apoptotic index in NM-3 plus carboplatin group was 3.5 times that of carboplatin control group (TUNEL: 27.98±6.12 % VS 12.94±2.12 %, FACScan: 26.86±5.69 % VS11.86±1.09 %,P0.01). Western blot analysis showed that the apoptotic index of human gastric cancer was elevated for 12, 24 and 36 h with an evident time-effect relationship in groups at 100 mg.L-L. NM-3 enhanced the inhibitive effects and sensitivity of chemotherapy for human gastric cancer in nude mice. These results suggested that NM-3 played a key inhibitive role in the growth of grafted human gastric cancer in nude ***: NM-3 can inhibit the growth of human gastric cancer cell line SGC-7901, and enhance the sensitivity of carboplatin on SGC-7901 and induced its apoptosis.