Expression of octopaminergic receptor genes in 4 nonneural tissues in female Nicrophorus vespilloides beetles
Expression of octopaminergic receptor genes in 4 nonneural tissues in female Nicrophorus vespilloides beetles作者机构：Department of Genetics University of Georgia Athens GA 30602 USA
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2015年第22卷第4期
页 面：495-502页
核心收录：
学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 0905[农学-畜牧学] 09[农学] 071006[理学-神经生物学] 0904[农学-植物保护] 0901[农学-作物学] 0713[理学-生态学]
基 金：University of Georgia's Office of the Vice President for Research Provost of the University of Georgia University of Georgia Center for Undergraduate Research Opportunities summer fellowship
主 题：biogenic amines Coleoptera G-protein coupled receptors quantitative real-time PCR
摘 要：Octopamine regulates the function of many tissues and physiological processes in invertebrates. The expression of octopamine receptor genes has been examined in multiple tissue types in several different insect orders. However, little work has addressed this issue in Coleoptera. Most studies characterize individual genes in different tissue types, but here we describe the expression of 6 octopamine receptor genes in thoracic musculature, oviducts, Malpighian tubules, and fat body of female Nicrophorus vespilloides beetles to characterize both different genes and different tissues within a single study. We then compare the gene expression profiles found in this beetle to other insects to examine the extent to which expression profiles are conserved across insects. We also examine the relative involvement of octopamine verses octopamine/tyramine receptors based on receptor gene expression in each tissue to help elucidate if tyramine plays a role in the regulation of these tissues. We find a high degree of overlap in the expression profile of the 6 genes examined in the thoracic muscula^tre, a moderate amount for the oviducts, and divergent profiles for Malpighian tubules and fat body. Based on expression difference in receptor subtypes, our results also support the suggestion that tyramine is a biogenic amine with physiological actions separate from octopamine.
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