A Set of SCAR Markers Efficiently Differentiating Hybrid Rice

作     者：LI Shu-jing XIE Hong-wei QIAN Ming-juan CHEN Guang-hui LI Shao-qing ZHU Ying-guo 

作者机构：State Key Laboratory of Hybrid Rice Key Laboratory for Research and Utilization of Heterosis in lndica Rice Ministry of Agriculture Engineering Research Center for Plant Biotechology and Germplasm Utilization of Ministry of Education College of Life Sciences Wuhan University Wuhan 430072 China Bangbu Medical College Bangbu 233030 China College of Plant Science Hunan Agricultural University Changsha 410128 China 

出 版 物：《Rice science》 (水稻科学（英文版）)

年 卷 期：2012年第19卷第1期

页      面：14-20页

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 0902[农学-园艺学] 

基　　金：the National High Technology Research and Development Program of China (Grant Nos. 2010AA101304 and 2008ZX08001-004) 

主　　题：hybrid rice molecular marker sequence characterized amplified region variety identification 

摘      要：Molecular markers have been widely used in crop genetic improvement, seed test and genetic mapping. Of which, sequence characterized amplified region （SCAR） markers are particularly popular for its diversity, stable reproducibility, and suitability for analyzing large number of samples. In this study, 500 random amplified polymorphic DNA （RAPD） primers were tested, and a set of SCAR markers comprising 37 pairs of loci-specific primers were developed from the DNA fragments ranging from 300 to 1000 bp which correspond to the stable, distinctive RAPD banding patterns. Using these SCAR markers, 59 hybrid rice combinations were assessed and distinguished into 58 subgroups at the similarity coefficient of 0.97 in a genetic clustering tree based on the allele diversities of the SCAR markers. Furthermore, 13 hybrid rice combinations were reassayed with 40 randomly selected simple sequence repeat （SSR） markers to evaluate the effectiveness of these SCAR markers. SSR markers produced similar results to SCAR markers as the 13 hybrid rice combinations were completely separated at the similarity coefficient of 0.91 in the clustering tree established from SSR patterns. Taken together, SCAR markers prove to be effective tools for identifying and differentiating hybrid rice combinations.