Optimization of L(+)-Lactic Acid Production from Xylose with Rhizopus Oryzae Mutant RLC41-6 Breeding by Low-Energy Ion Implantation

作     者：杨英歌 樊永红 李文 王冬梅 吴跃进 郑之明 余增亮 

作者机构：Key Laboratory of Ion Beam BioengineeringChinese Academy of SciencesHefei 230031China Bioengineering high vocational school of XuzhouXuzhou 221006China Key Laboratory of Ion Beam BioengineeringChinese Academy of SciencesHefei 230031China 

出 版 物：《Plasma Science and Technology》 (等离子体科学和技术（英文版）)

年 卷 期：2007年第9卷第5期

页      面：638-642页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学] 

基　　金：supported by the Key‘863’Fund of China (No.2006AA020102) Key Technology Research and Development Program of Anhui Province in 2007 (No.07010202076) 

主　　题：L(+)-lactic acid xylose Rhizopus oryzae ion implantation 

摘      要：In order to obtain an industrial strain with a higher L（＋）-lactic acid yield, the strain Rhizopus oryzae PW352 was mutated by means of nitrogen ion beam implantation and the mutant strain Rhizopus oryzae RLC41-6 was obtained. An experimental finding was made in surprise that Rhizopus oryzae mutant RLC41-6 is not only an L（＋）-lactic acid producer from corn starch but also an efficient producer of L（＋）-lactic acid from xylose. Under optimal conditions, the production of L（＋）-lactic acid from 100 g/L xylose reached 77.39 g/L after 144 h fed-batch fermentation, A high mutation rate and a wide mutation spectrum of low-energy ion implantation were observed in the experiment.