Warthin-starry Silver Method Showing Particulate Matter in Macrophage

作     者：HONG-GANG LIU 

作者机构：Department of Pathology Beijing Tongren Hospital Capital Medical University Beijing 100730 China 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2008年第21卷第1期

页      面：85-89页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

基　　金：the Electively Sponsored Project for Scientific Research of Returnees After Study Abroad 

主　　题：Fine particulate matter Warthin-Starry stains Macrophage Dust cell 

摘      要：Objective To verify whether Warthin-Starry （WS） silver method could detect the air particulate matter （PM）/dust particles （Ps） located within the macrophages in situ. Methods There were 26 autopsy cases that resulted from cerebral hemorrhage （group A）, silicosis （group B）, and fetal death during pregnancy （group C）. Samples were collected separately and serial sections were prepared from the lungs and lymph nodes and stained with hematoxylin and eosin （HE）, WS silver, immunohistochemistry of CD68. Furthermore, ultrathin sections were taken from the WS positive serial sections of groups A and B. Ps were observed under a transmission electron microscope （TEM） and the elements of Ps were measured by X-ray spectrum analysis （X-RSA）. Results In both groups A and B, WS staining was positive for the larger and fine Ps, the so called ＂dust cells＂, but HE staining was almost negative for fine Ps. In group C, no larger or fine Ps were found. Immunohistochemical staining of CD68 certified that the ＂dust cells＂ containing Ps were macrophages. The results of TEM and X-RSA proved that the structure and elements of Ps belonged to PM indeed. Conclusion WS staining is a better than HE staining in showing the location of PM within macrophages.