Flow-injection Spectrophotometric Determination of Uric Acid in Urine via Prussian Blue Reaction

作     者：WASEEM Amir YAQOOB Mohammad NABI Abdul MURTAZA Ghulam HUSSAIN Izhar 

作者机构：Department of Chemistry COMSATS Institute of Information Technology Department of Chemistry University of Balochistan Department of Pharmacy COMSATS Institute of Information Technology 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)

年 卷 期：2011年第27卷第6期

页      面：929-933页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 1010[医学-医学技术（可授医学、理学学位）] 070302[理学-分析化学] 0703[理学-化学] 10[医学] 

基　　金：Supported by the CIIT-Project Funded by COMSATS Institute of Information Technology Pakistan 

主　　题：Flow injection analysis Spectrophotometry Uric acid Prussian blue Urine 

摘      要：A simple and sensitive flow injection（FI） spectrophotometric method was reported for the determination of uric acid based on the reduction of Fe（III）/ferricyanide in the presence of uric acid. The in situ reduced ions reacted with unreduced portion of ferricyanide/Fe（III） to form soluble Prussian blue, which was monitored at an absorption wavelength of 735 nm. The optimized conditions allow a linear calibration graph in a concentration range of 1―100 μmol/L. The relative standard deviation was in a range of 0.5%―2.5%, with a detection limit（3σ blank） of 0.3 μmol/L and a sampling frequency of 60 injection/h was obtained. The effect of common substances present in human physiological fluids on the determination of uric acid was examined. The method was applied to determining uric acid in human urine samples with the recoveries in a range of 96%―105%. The results agree well with those by spectrophotometric reference method at a confidence level of 95%. Spectrophotometric procedures for uric acid determination in clinical samples were reviewed briefly.