Effect of cefodizime on cytokines expression in mouse neutrophils and its related mechanism

作     者：Song Hui Qian Yuanshu Li Guanwu 

作者机构：Department of Pharmacology Medical College Shantou University Shantou 515041 China Guangdong Food and Drug Vocational College Guangzhou 515041 China Department of Molecular Biologv Medical College Shantou University Shantou 515041 China 

出 版 物：《Journal of Medical Colleges of PLA(China)》 (中国人民解放军军医大学学报（英文版）)

年 卷 期：2008年第23卷第4期

页      面：243-250页

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

基　　金：the Natural Science Foundation of GuangdongProvince (5300983) 

主　　题：中性白细胞 TNF-α IL-1β 核因子 细胞活素 

摘      要：Objective: To explore the regulating effects of cefodizime on cytokines expression of neutrophil response to Klebsiella pneumoniae (Kle. p) treatment. Methods: We detected the types and expression of cytokines secreted by neutrophils by cDNA array and RT-PCR. We also analyzed the changes of signal transduction in this process by detecting the expression of toll like receptor 4 (TLR4) and the inhibitor factor of κBα (I-κBα) expressed by neutrophils. The activity of NF-κB DNA-binding in neutrophils was measured by electrophoretic mobility shift assay (EMSA). Results: Cefodizime increased the neutrophils production of TNF-α, IL-1β and the mRNA expression of TLR4 in the early stage of Kle. p stimulation in mice, which seemed corresponding to the enhanced NF-κB DNA-binding activity. Conclusion: Cefodizime regulates the cytokines expression of neutrophils through the LPS-TLR4-NF-κB pathway by affecting the expression of TLR4 mRNA and the DNA binding activities of NF-κB in mice with the challenge of Kle. p.