Simple and efficient delivery of cellimpermeable organic fluorescent probes into live cells for live-cell superresolution imaging
作者机构：Britton Chance Center for Biomedical PhotonicsWuhan National Laboratory for Optoelectronics-Huazhong University of Science and TechnologyWuhanHubei 430074China MoE Key Laboratory for Biomedical PhotonicsSchool of Engineering SciencesHuazhong University of Science and TechnologyWuhanHubei 430074China
出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)
年 卷 期：2019年第8卷第1期
页 面：563-573页
核心收录：
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学]
基 金：supported by the National Basic Research Program of China(Grant No.2015CB352002) the National Natural Science Foundation of China(Grant No.31770924,61827825,31600692,31470056) the Science Fund for Creative Research Group of China(Grant No.61721092) the Director Fund of the Wuhan National Laboratory for Optoelectronics
主 题：resolution allowed render
摘 要：Numerous commercial organic fluorophores with excellent optical properties are precluded from live-cell superresolution imaging due to poor cell ***,we develop a simple but effective strategy that renders cells permeable to cell-impermeable,organic fluorescent probes by using a novel peptide vehicle,*** simple coincubation with PV-1,22 different cell-impermeable,organic fluorescent probes were efficiently delivered into live cells and specifically labeled a variety of ***,PV-1 can simultaneously transfer up to three different probes into live *** using PV-1 and these cell-impermeable fluorescent probes,we obtained multicolor,longterm,live-cell superresolution images of various organelles,which allowed us to study the dynamic interactions between ***-1,together with these organic fluorescent probes,will greatly broaden the applications of superresolution imaging technology in diverse live-cell studies and opens up a new avenue in the design and application of peptide vehicles.
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