Influence of different distinct differentiation of trophoblastic cells on binding characteristics of targeted microbubble contrast agents in vitro

作     者：Lixue Zhou Ruike Pan Xin Zhang Lirong He 

作者机构：Department of Gynecology and Obstetrics The Second Affiliated Hospital Sun Yat-sen University Guangzhou 510120China 

出 版 物：《The Chinese-German Journal of Clinical Oncology》 (中德临床肿瘤学杂志（英文版）)

年 卷 期：2010年第9卷第4期

页      面：229-234页

学科分类：1002[医学-临床医学] 10[医学] 

基　　金：Supported by a grant from the Science-Technology Program Foundation of Guangdong Province China (No.2006B35901009) 

主　　题：sonoVue microbubbles trophoblast cells choriocarcinoma cells distinct differentiation 

摘      要：Objective:In this study, we investigated the in vitro binding capacity of β-HCG antibody targeted SonoVue microbubbles to trophoblasts with distinct differentiation in order to explore the possibility of utility of targeted SonoVue microbubbles imaging for early locating diagnosis of malignant trophoblastic cell disease. Methods:Three cell groups were included in the study: (1) choriocarcinoma cells (poorly differentiated) (JAR, n=10), (2) early gestational trophoblastic cells (ETC, n=10) and (3) late placenta trophoblastic cells (LTC, n=10). The binding efficiency of the contrast agents to the targeted cells was evaluated by counting the ring formation rate before and after rinsing with PBS. Results: The binding rate was significantly higher in JAR group 84.3±5.5% than in the ETC group 67.3±3.9% and LTC group 60.4±4.6% (P0.05). The binding rates of the targeted microbubbles labeled with fluorescence by FCM to JARs, ETC or LTC were 90.1%, 81.5% and 69.2%, respectively (P0.05). Conclusion: This in vitro study demonstrated that β-HCG an-tibody-targeted SonoVue had different binding capacities to trophoblasts with distinct degrees of differentiation. The highest binding rate occurred with the choriocarcinoma cell line JAR. There is the possibility that the β-HCG antibody-targeted strategy could improve the discriminative ability of SonoVue, in the locating malignant trophoblastic cells.