Role of Wnt Inhibitory Factor-1 in Inhibition of Bisdemethoxycurcumin Mediated Epithelial-to-Mesenchymal Transition in Highly Metastatic Lung Cancer 95D Cells

作     者：Jin-Hong Xu He-Ping Yang Xiang-Dong Zhou Hai-Jing Wang Liang Gong Chun-Lan Tang Xu Jin-Hong;Yang He-Ping;Zhou Xiang-Dong;Wang Hai-Jing;Gong Liang;Tang Chun-Lan

作者机构：Department of Respiratory Medicine Southwest Hospital Third Military Medical University Chongqing 400038 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2015年第128卷第10期

页      面：1376-1383页

核心收录：

学科分类：0710[理学-生物学] 090602[农学-预防兽医学] 1002[医学-临床医学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0906[农学-兽医学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

主　　题：Bisdemethoxycurcumin Epithelial-to-mesenchymal Transition Lung Cancer Wnt Inhibitory Factor- 1 Wnt Pathway 

摘      要：Background：Bisdemethoxycurcumin （BDMC） is an active component of curcumin and a chemotherapeutic agent,which has been suggested to inhibit tumor growth,invasion and metastasis in multiple *** its contribution and mechanism of action in invasion and metastasis of non-small cell lung cancer （NSCLC） are not very ***,we tried to study the effects of BDMC on regulation of epithelial-to-mesenchymal transition （EMT）,which is closely linked to tumor cell invasion and ***：In this study,we first induced transforming growth factor-βl （TGF-β1） mediated EMT in highly metastatic lung cancer 95D ***,we studied the effects of BDMC on invasion and migration of 95D *** addition,EMT markers expressions were also analyzed by western blot and immunofluorescence *** contribution of Wnt inhibitory factor-1 （WIF-1） in regulating BDMC effects on TGF-β1 induced EMT were further analyzed by its overexpression and small interfering RNA knockdown ***：It was observed that BDMC inhibited the TGF-β1 induced EMT in 95D ***,it also inhibited the Wnt signaling pathway by upregulating WIF-1 protein *** addition,WIF-1 manipulation studies further revealed that WIF-1 is a central molecule mediating BDMC response towards TGF-β1 induced EMT by regulating cell invasion and ***：Our study concluded that BDMC effects on TGF-β1 induced EMT in NSCLC are mediated through WIF-1 and elucidated a novel mechanism of EMT regulation by BDMC.