High-Molecular-Weight Glutenin Subunit Composition of Chinese Wheat Germplasm
High-Molecular-Weight Glutenin Subunit Composition of Chinese Wheat Germplasm作者机构：College of Agronomy Northwest A＆F University Yangling 712100 P.R.China The National Key Facilities for Crop Genetic Resources and Improvement （NFCRI） Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China
出 版 物：《Agricultural Sciences in China》 (中国农业科学（英文版）)
年 卷 期：2007年第6卷第8期
页 面：899-907页
基 金：Key Project of 10th Five-Year Plan Ministry of Science and Technology of the People's Republic of China, MOST, (2004BA525B03) Ministry of Science and Technology of the People's Republic of China, MOST
主 题：bread wheat high-molecular-weight glutenin subunits (HMW-GS) 1B/1R translocation
摘 要：The objective of the present study was to characterize the high molecular glutenin subunits （HMW-GS） composition and the presence of 1B/1R translocation in newly developed wheat （Triticum aestivum L.） germplasm, which have one or more traits that are useful in wheat improvement. Sodium dodecyl sulphate polyacrylamide-gel electrophoresis （SDS-PAGE） and acid polyacrylamide-gel electrophoresis （A-PAGE） were used to detect HMW-GS composition and the presence of 1B/1R wheat-rye （Secale cereale L.） chromosome translocation in the wheat germplasm. Bread-making quality scores of these lines were determined. A high level of variations in HMW-GS encoded by Glu-1 locus was observed. Sixteen major HMW-GS, with 30 combinations, were detected. The percentage of cultivars with more than two desirable subunits was 38.7%. Thirteen cultivars had bread-making quality scores of 10 in combination with one or two desirable agronomical traits, such as high-yield potential, dwarfing stem, resistance to diseases, and/or tolerance to abiotic stress. Sixty-eight （36.6%） cultivars possessed 1B/1R translocation. The newly developed germplasm with HMW-GS for good quality can be promising resources for improving bread-making quality of wheat.
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