Deactivation Kinetics of Nitrile Hydratase in Free Resting Cells
Deactivation Kinetics of Nitrile Hydratase in Free Resting Cells作者机构：Department of Chemical Engineering Tsinghua University Beijing 100084 China
出 版 物：《Chinese Journal of Chemical Engineering》 (中国化学工程学报（英文版）)
年 卷 期：2009年第17卷第5期
页 面：822-828页
核心收录：
学科分类：0710[理学-生物学] 081703[工学-生物化工] 07[理学] 08[工学] 0817[工学-化学工程与技术] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 082203[工学-发酵工程] 090102[农学-作物遗传育种] 0822[工学-轻工技术与工程]
基 金：Supported by the Foundation for the Authors of National Excellent Doctoral Dissertation of China (200345) the National High Technology Research and Development Program of China (2007AA02Z201) the National Basic Research Program of China (2007CB714304)
主 题：nitrile hydratase deactivation kinetics free resting cell bi-steady state assumption total deactivation rate constant
摘 要：Nitrile hydratase (NHase) is an important industrial enzyme used for acrylamide production from *** deactivation kinetics of NHases in free resting cells of Rhodococcus *** presented based on a bi-steady state *** of hydration temperature,product concentration and substrate concentration on NHase deactivation were investigated experimentally and correlated with a first order deactivation *** results showed that the hydration temperature and product concentration were major factors governing the deactivation of NHases under substrate-feeding *** acrylamide concentration was higher than 250 g·L1,the deactivation of NHases became serious and the bi-steady state assumption was not *** the hydration temperature was controlled at a relatively higher level such as 28°C,the total deactivation rate constant was about 2.8-fold of that at 20°C.
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