Shrinkage of ipsilateral taste buds and hyperplasia of contralateral taste buds following chorda tympani nerve transection

作     者：Yi-ke Li Juan-mei Yang Yi-bo Huang Dong-dong Ren Fang-lu Chi 

作者机构：Department of Otology and Skull Base SurgeryEye Ear Nose & Throat HospitalFudan University Shanghai Auditory Medical Center Key Laboratory of Hearing ScienceMinistry of Health Department of Hearing and Speech SciencesVanderbilt University 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2015年第10卷第6期

页      面：989-995页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：supported by the Major State Basic Research Development Program of China(973 Program),No.2011CB504500,2011CB504506 the National Natural Science Foundation of China,No.81271084,81420108010,81000413,81370022,81200740 the Training Program of the Excellent Young Talents of the Shanghai Municipal Health System in China,No.XYQ2013084 the Innovation Project of Shanghai Municipal Science and Technology Commission in China,No.11411952300 

主　　题：nerve regeneration gustation cytokeratin tongue epithelium immunohistochemistry taste bud trigeminal nerve disorder NSFC grants neural regeneration 

摘      要：The morphological changes that occur in the taste buds after denervation are not well under- stood in rats, especially in the contralateral tongue epithelium. In this study, we investigated the time course of morphological changes in the taste buds following unilateral nerve transection. The role of the trigeminal component of the lingual nerve in maintaining the structural integrity of the taste buds was also examined. Twenty-four Sprague-Dawley rats were randomly divided into three groups： control, unilateral chorda tympani nerve transection and unilateral chorda tympani nerve transection ＋ lingual nerve transection. Rats were allowed up to 42 days of re- covery before being euthanized. The taste buds were visualized using a cytokeratin 8 antibody. Taste bud counts, volumes and taste receptor cell numbers were quantified and compared among groups. No significant difference was detected between the chorda tympani nerve transection and chorda tympani nerve transection ＋ lingual nerve transection groups. Taste bud counts, volumes and taste receptor cell numbers on the ipsilateral side all decreased significantly compared with control. On the contralateral side, the number of taste buds remained unchanged over time, but they were larger, and taste receptor cells were more numerous postoperatively. There was no evidence for a role of the trigeminal branch of the lingual nerve in maintaining the structural integrity of the anterior taste buds.