DAPT Enhances the Apoptosis of Human Tongue Carcinoma Cells

作     者：Brian E. Grottkau Xi-rui Chen Claudia C. Friedrich Xing-mei Yang Wei Jing Yao Wu Xiao-xiao Cai Yu-rong Liu Yuan-ding Huang Yun-feng Lin 

作者机构：Department of Orthopaedic Surgery MassGeneral Hospital for Children and the Pediatric Orthopaedic Laboratory for Tissue Engineering Harvard Medical School Boston USA State Key Laboratory of Oral Diseases Sichuan University Chengdu China Institute of Technology University of Toronto Ontario Canada Department of Oral and Maxillofacial Surgery Beijing Friendship Hospital affiliated to Capital Medicine UniversityBeijing China 

出 版 物：《International Journal of Oral Science》 (国际口腔科学杂志（英文版）)

年 卷 期：2009年第1卷第2期

页      面：81-89页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：funded by the National Natural Science Foundation of China(30801304) Specialized Research Fund for the Doctoral Program of Higher Education(20070610062) Opening Funding of the State Key Laboratory of Oral Diseases, Sichuan University(SKLOD011) the Applied Fundarmental Project of Sichuan Province(2008 JY0028-2) 

主　　题：DAPT human tongue carcinoma cells Notch,Caspase-3 

摘      要：Aim To investigate the effect of DAPT （γ-secretase inhibitor） on the growth of human tongue carcinoma cells and to determine the molecular mechanism to enable the potential application of DAPT to the treatment of tongue carcinoma. Methodology Human tongue carcinoma Tca8113 cells were cultured with DAPT. Cell growth was determined using Indigotic Reduction method. The cell cycle and apoptosis were analyzed by flow cytometry. Real-time PCR and Immuno-Fluorescence （IF） were employed to determine the intracellular expression levels. Results DAPT inhibited the growth of human tongue carcinoma Tca8113 cells by inducing G0-G1 cell cycle arrest and apoptosis, The mRNA levels of Hairy/Enhancer of Split-1 （Hes-1）, a target of Notch activation, were reduced by DAPT in a dose-dependent manner. Coincident with this observation, DAPT induced a dose-dependent promotion of constitutive Caspase-3 in Tca8113 cells. Conclusion DAPT may have a therapeutic value for human tongue carcinoma. Moreover, the effects of DAPT in tumor inhibition may arise partly via the modulation of Notch- 1 and Caspase-3.