Vitamin B2 functionalized iron oxide nanozymes for mouth ulcer healing

作     者：Yunhao Gu Yixin Huang Zhiyue Qiu Zhuobin Xu Dandan Li Lei Chen Jing Jiang Lizeng Gao Yunhao Gu;Yixin Huang;Zhiyue Qiu;Zhuobin Xu;Dandan Li;Lei Chen;Jing Jiang;Lizeng Gao

作者机构：Institute of Translational MedicineDepartment of PharmacologySchool of MedicineYangzhou UniversityYangzhou 225001China Jiangsu Key Laboratory of Experimental and Translational Non-coding RNA ResearchYangzhou UniversityYangzhou 225001China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2020年第63卷第1期

页      面：68-79页

核心收录：

学科分类：1003[医学-口腔医学] 100302[医学-口腔临床医学] 10[医学] 

基　　金：supported by the National Key Research and Development Program of China(2018YFC1003500) the National Natural Science Foundation of China(81671810 and 31701236) the Jiangsu Provincial Basic Research Program for the Natural Science Foundation(BK20161333) 

主　　题：iron oxide nanozymes vitamin B2 ROS scavenging mouth ulcer anti-inflammation 

摘      要：Mouth ulcer is associated with inflammation and high risk of bacterial infection, which aggravates the patient s ***, there is no effective treatment for mouth ulcer. Herein, we report that vitamin-modified iron oxide nanoparticles improve the healing of mouth ulcer through anti-inflammation and antibacterial activities. We discovered that vitamin B2(VB2)modified iron oxide nanoparticles performed enhanced peroxidase-like, catalase-like, and superoxide dismutase(SOD)-like activities, acting as typical iron oxide nanozymes(IONzymes) with triad activities. In particular, VB2 modification significantly improved the SOD-like activity, thus providing a reactive oxygen species(ROS)-scavenging ability. Cellular antioxidant experiments showed that vitamin B2 modified IONzymes(VB2-IONzymes) protect human oral keratinocytes(HOK) and BALB/3 T3 cells from hydrogen peroxide(H2O2), and these cells have high biocompatibility to eukaryotic cells. In addition, VB2-IONzymes exerted an antibacterial activity against Streptococcus mutans, Staphylococcus aureus, and Escherichia coli. Importantly, VB2-IONzymes accelerated the recovery of mouth ulcer and reduced the local secretion of inflammatory factors in mouse ulcer model via ROS scavenging and antibacterial activity. Taken together, our work demonstrates that vitamin B2 modification endows iron oxide nanoparticles with enhanced enzyme-like activities and VB2-IONzymes may be a promising reagent in the treatment of mouth ulcer because of their intrinsic anti-inflammation and antibacterial capabilities.