β-Cyclodextrin polymer based fluorescence enhancement method for sensitive adenosine triphosphate detection

作     者：Chunxia Song Yuxin Xiao Kunpeng Li Xiaoyu Zhang Ying Lu 

作者机构：Department of Applied Chemistry School of Science Anhui Agricultural University 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2019年第30卷第6期

页      面：1249-1252页

核心收录：

学科分类：07[理学] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：financially supported by the National Natural Science Foundation of China (Nos. 21305002 and 21705002) the Scientific Research Foundation of Anhui Agricultural University (No. yj2017-19) 

主　　题：β-Cyclodextrin polymer Supramolecular assembly Fluorescence Enhancement Adenosine triphosphate 

摘      要：Adenosine triphosphate plays a crucial role in regulation of many biological pathways and has been used as an indicator for many diseases.In this pape r,based on the fact that β-cyclodextrin polymer(polyβ-CD)could significantly enhance pyrene fluorescence through supramolecular assembly(host-gest interaction),a sensitive and facile method for adenosine triphosphate detection has been developed.A 3’-pyrene-labelled ATP-binding aptamer was employed as the fluorescence probe,which could be digested by exonuclease I to obtain mononucleotides,with pyrene attached on.The pyrene attached on mononucleotides could easily enter the hydrophobic cavity of polyβ-CD,accompanied with prominent fluo rescence enhancement.While ATP was introduced,ATP and its aptamer could combine together and the obtained hairpin complex could not be cleaved by exonuclease Ⅰ,The pyrene labelled on the probe could not enter the cavity of polyβ-CD belong to the complex’steric hindrance,accompanied with the weak pyrene’fluorescence.So we could quantify the concentration of adenosine triphosphate facilely by measuring the fluorescence intensity of the system.The detection limit of this method for adenosine triphosphate was 11 μmol/L(S/N=3),The developed method showed sufficient selectivity and could successfully assay adenosine triphosphate in biological samples.The developed method provides a potential platform for biological micromoles assay.